THE ACTIVATION ENERGY OF UREA HYDROLYSIS CATALYZED BY SOY BEAN UREASE

The Activation Energy of Urea Hydrolysis Catalyzed by Soy Bean Urease

The rate of most chemical reactions increases exponentially with temperature in accordance with the Arrhenius equation. The older literature on enzyme-catalyzed reactions (for references cf. Haldane (1930), Tauber (1937)) indicates that these do not. conform, since the activation energy decreases with rise in temperature instead of being invariant. More recent studies do not confirm this genera...

The competitive inhibition of the urease-catalyzed hydrolysis of urea by phosphate.

It follows from equation (1) that when l/u is plotted (usually as the ordinate) against l/s a straight line will be obtained with inhibitory action influencing either the slope or the ordinate intercept or both. Thus the type of inhibition may be defined on the basis of the effect of the inhibitory action upon the slope and intercept in the above plot. In the absence of an inhibitor (i = 0) equ...

The Reversibility of the Action of Urease of Soy Bean.

Studies of the Intermediate Products Formed during the Hydrolysis of Urea by Urease”

In 1923, Fearonl stated that he had been able to detect traces of cyanic acid in solutions in which urea was being decomposed by soy bean urease. He proposed that the reaction concerned is not a true hydrolysis, but rather a cleavage of the urea molecule into cyanic acid and ammonia and that the cyanic acid is at once spontaneously hydrolyzed to ammonia and carbon dioxide.2 Sumner,3 in 1926, ob...

Activation of the Urease - Urea System Using Crude and Crystalline

Many studies of the kinetics of enzyme action as a function of temperature have been made. The older work (for reference cf. Haldane, 1930; Tauber, 1937) indicates that while the velocity of an enzyme-catalyzed reaction increases with rise in temperature, up to the inactivation temperature of the enzyme, this change in rate is not an exponential function of the absolute temperature. I t has bee...